human colon cancer epithelial cell line hct 116 Search Results


hct116 human colonic epithelial cells  (ATCC)
99
ATCC hct116 human colonic epithelial cells
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Hct116 Human Colonic Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela cells  (ATCC)
97
ATCC hela cells
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fibroblast growth medium fgm  (Cell Applications Inc)
96
Cell Applications Inc fibroblast growth medium fgm
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Fibroblast Growth Medium Fgm, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human colon carcinoma cell lines hct 116  (ATCC)
99
ATCC human colon carcinoma cell lines hct 116
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Human Colon Carcinoma Cell Lines Hct 116, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human colon cancer  (ATCC)
99
ATCC human colon cancer
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Human Colon Cancer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hct-116 cell line  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare hct-116 cell line
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Hct 116 Cell Line, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hct 116  (DSMZ)
95
DSMZ hct 116
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Hct 116, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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experimental models htert rpe 1 human atcc crl 4000 hct 116  (ATCC)
99
ATCC experimental models htert rpe 1 human atcc crl 4000 hct 116
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Experimental Models Htert Rpe 1 Human Atcc Crl 4000 Hct 116, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cancer cell lines  (ATCC)
99
ATCC cancer cell lines
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cancer cell lines - by Bioz Stars, 2026-07
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glycerophosphocholine  (Cell Applications Inc)
96
Cell Applications Inc glycerophosphocholine
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Glycerophosphocholine, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hct-116  (CEM Corporation)
90
CEM Corporation hct-116
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Hct 116, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colon carcinoma cells hct 116 anticancer  (ATCC)
99
ATCC colon carcinoma cells hct 116 anticancer
(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into <t>HCT116</t> epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).
Colon Carcinoma Cells Hct 116 Anticancer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colon carcinoma cells hct 116 anticancer - by Bioz Stars, 2026-07
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Image Search Results


(A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into HCT116 epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).

Journal: bioRxiv

Article Title: Non-redundant dimethyl sulfoxide reductases influence Salmonella enterica serotype Typhimurium anaerobic growth and virulence

doi: 10.1101/2022.11.29.517730

Figure Lengend Snippet: (A, D) Overnight cultures of the indicated bacterial strains bearing a P prgH - lacZY transcriptional fusion were diluted into LB and grown aerobically at 37°C. Where indicated, LB was supplemented with 1% (v/v) DMSO or DMS after 1.5 hours growth and incubation continued for another 1.5 hours. The activity of the prgH promoter was estimated by measuring β-galactosidase activity, which was converted into Miller Units. (B) Bacteria were prepared as in (A) and invasion efficiency into HCT116 epithelial cell monolayers was determined by gentamicin protection assay at 1 hour post-infection. Invasion efficiency was normalized to the untreated WT strain. (B) HCT116 cells were infected with the indicated bacterial strains (constitutively expressing mCherry ) bearing a P prgH -GFP[LVA] transcriptional fusion. Cells were fixed at the indicated timepoints, and the proportion of GFP-positive bacteria quantified by fluorescence microscopy. Bars represent the mean +/- SEM of 3 (A, C, D) or 4 (B) independent experiments. Asterisks (*) indicate significant difference from media alone as detected by Student’s t-test (p<0.05).

Article Snippet: HCT116 (human colonic epithelial) cells and J774A.1 (mouse macrophage-like) cells were purchased from ATCC and maintained in McCoy’s 5A media (Corning) containing 10% heat-inactivated fetal calf serum (FCS, Invitrogen) or Dulbecco’s Modified Eagle’s medium (Corning) containing 10% heat-inactivated FCS, respectively.

Techniques: Incubation, Activity Assay, Bacteria, Infection, Expressing, Fluorescence, Microscopy